DEAGON Casandra, Amgen, USA
REYES Anel, Amgen, USA
CRAIG Amanda, Amgen, USA
GARCIA MENENDEZ Lorena, Amgen, USA
Animal research facilities committed to a culture of care are increasingly developing structured programs that support lifelong welfare beyond research endpoints. We present a four-week canine acclimation and socialization program that prepares research animals for successful adoption by systematically desensitizing them to household stimuli while reinforcing positive behavior. Building upon compatibility assessments and ethogram-guided pairings at intake, our poststudy program utilizes daily interactions, positive reinforcement, and exposure to novel environments, sounds, and social situations.
Canines engage in gradual training that includes elevator use, stairs, toilet sounds, carrier time, and basic hand-signal commands such as sit, stay, and come. Weekly routines introduce new food items and simulate real-world home environments with structured increases in duration and complexity. Socialization is further enriched through supervised play sessions and daily volunteer-supported interactions. Data from these sessions guide individualized approaches that support animal confidence and reduce stress.
The program has led to smoother behavioral transitions post-adoption, with early adopters reporting successful acclimation in home settings. Celebrating program milestones—such as “Top Dog” graduation events—also fosters staff engagement and reinforces institutional commitment to animal welfare.
This initiative demonstrates how a structured, behaviorally-informed acclimation program not only enhances postresearch life for animals but also strengthens institutional culture of care and cross-functional collaboration in research settings.
Carly I. O'Malley, Charles River, USA
Emilie A. Paterson, Charles River, USA
Dawn M. Abney, Charles River, USA
William J. Archibald, Charles River, UK
Sarah E. Thurston, Charles River, USA
Patricia V. Turner, Charles River, Canada
Elizabeth A. Nunamaker. Charles River, USA
Animal welfare is a vital component of the work we do with research animals as not only is it ethical to provide the best care and management we can for the animals, but it also improves research validity and translatability. Across research organizations, or even within the same organization it can be difficult to standardize welfare criteria and assessment. Implementing welfare assessment tools provides a means of harmonizing welfare assessment methods across facilities, and allows for on-going, consistent, and holistic assessment of animal programs, creating a common language for discussion and evaluation. Charles River has developed and launched species-specific welfare assessment tools through formation of stakeholder focus groups of species experts, identification of welfare categories and descriptors, developing preliminary tools for beta testing at global facilities, and finally building and launching automated tools that provide summarized results to personnel. The welfare categories include physical, behavioral, environmental, training, procedural, and culture of care. Personnel at each facility analyze the results of their reports, have discussions of available resources and priorities, and reach consensus on yearly goals. Feedback on the tools indicate that personnel find the tools valuable in assessing their programs and that the reports and goal setting help sites prioritize projects. It has also helped highlight barriers to program improvement, such as workload, staffing, and training personnel. To date, primate, dog, and rabbit tools have been provided to Charles River facilities, with pig, rat, and mouse tools in development.
CRIM Marcus J., IDEXX BioAnalytics, Columbia, MO, USA
HART Marcia L., IDEXX BioAnalytics, Columbia, MO, USA
HANSEN Sarah A., IDEXX BioAnalytics, Columbia, MO, USA
LIVINGSTON Robert S., IDEXX BioAnalytics, Columbia, MO, USA
Environmental enrichment aims to improve animal welfare. However, some types of environmental enrichment for zebrafish are controversial because of limited information in the scientific literature, as well as potential biosecurity risks and challenges related to the logistics of implementation. Nevertheless, most studies assessing zebrafish preferences or testing the impact of conditions intended to provide enrichment suggest or support a benefit to zebrafish welfare. We therefore employ several types of environmental enrichment in our small zebrafish colony, including physical as well as social and sensory enrichment. Physical enrichment includes the routine use of plastic plants that span the majority of the water column and/or PVC structures. Physical enrichment is used with social housing, live feed, and pebble picture sensory enrichment to provide a more complex environment than commonly used barren tanks, more closely mimicking natural habitats in order to reduce stress and promote natural behaviors. This presentation will summarize the current literature surrounding zebrafish enrichment, give examples of our method of institution of enrichment and provide ideas for your own facility. It will be of interest to veterinarians, technicians, husbandry staff and IACUC members.
METZLER Marnie Silverstein, Office of Research and Innovation, North Carolina State University, Raleigh, NC, USA
MAHS Sina, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
KINLAW Sarah, Office of Research and Innovation, North Carolina State University, Raleigh, NC, USA
ESTES Jenny, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA
PARSONS Casey, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, NC, USA
TRIVEDI Shweta, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, NC, USA
Canine enrichment and socialization are vital components of canine health and welfare in a laboratory setting. While canines represent less than 1% of biomedical research, they remain a vital part of veterinary research and education. At North Carolina State University College of Veterinary Medicine, 20-30 beagle and mongrel hound dogs are each held for a 2-3 year period for veterinary instruction and minimally invasive research; at the end of this period they become available for adoption. In order to provide socialization and play, acclimate dogs to student teaching labs, and prepare dogs for adoption, the College of Veterinary Medicine, College of Agriculture and Life Sciences, and University Attending Veterinarian collaboratively formed Canine College. This program features a semester-long competitive internship which pairs pre-veterinary student interns with 4-6 dogs and culminates in a student-led project and presentation. Benefits to the dogs include 1-on-1 social interaction with their intern, outdoor play, training, and personalized enrichment delivered by the student in consultation with the Laboratory Animal Resource department's enrichment specialists. Students learn canine training; observe behavior; and engage with laboratory animal medicine, research, and scientific presentation. Internship projects have included behavioral ethogram surveys and adoption questionnaires. Through these projects we have identified common laboratory and in-home behavioral challenges including, anxiety, neophobia, stranger-danger, housebreaking, and ingestion of non-food items. This has allowed us to refine how we approach canine behavior in the laboratory setting while also fostering a culture of care as interns become ambassadors for laboratory animal science, comparative medicine, and translational research.
Pemika Vipabusabakorn, i-Cattery, i-Tail Corporation PCL, Nakhon Pathom, Thailand
Hatairat Khloikhiao, i-Cattery, i-Tail Corporation PCL, Nakhon Pathom, Thailand
Sirada Chuthep, i-Cattery, i-Tail Corporation PCL, Nakhon Pathom, Thailand
Watuka Wannarun, i-Cattery, i-Tail Corporation PCL, Nakhon Pathom, Thailand
Boonyarat Detruengsri, i-Cattery, i-Tail Corporation PCL, Nakhon Pathom, Thailand
Sisupa Pongsiwat, i-Cattery, i-Tail Corporation PCL, Nakhon Pathom, Thailand
As the only pet food manufacturer globally accredited by AAALAC International, i-Tail Corporation, through its i-Cattery facility in Thailand, prioritizes feline welfare in research. Managing cat behavior, particularly aggression and colony integration, presents challenges. Traditional methods caused stress, social conflicts, and prolonged adaptation, affecting welfare and research consistency.
To address this, i-Tail Corporation developed an advanced behavioral care protocol at i-Cattery, improving welfare and research reliability. This protocol reduces aggression and social incompatibility through strategic group separation, controlled exposure, and desensitization. Gradual introductions minimize stress, prevent conflicts, and foster stable bonds.
Environmental enrichment-including interactive play, climbing structures, and supervised socialization-supports natural behaviors and well-being. A specialized feline behavior team continuously monitors progress, adjusting care as needed. Regular veterinary assessments reinforce individualized care, enhancing health.
These innovations enable smoother group integration, reduced stress, and improved welfare. By adhering to AAALAC International guidelines and implementing a structured, humane approach, i-Cattery sets a standard for feline research. This protocol serves as a model for other facilities, providing insights into effective socialization and welfare strategies.
i-Cattery ensures cats spend at least six hours daily in an enriched playground. The space includes scratching posts, cat trees, and window perches. Collapsible tunnels encourage play, while a large window overlooking a horse field provides sensory enrichment. Cats are supervised, ensuring safety and natural behaviors. By integrating behavioral care, enrichment, and supervision, i-Tail Corporation enhances feline welfare and research integrity. These practices demonstrate measurable improvements in animal care and contribute to a broader Culture of Care worldwide.
Andrew T. Stevens, Western Michigan University Homer Stryker M.D. School of Medicine WE Upjohn Campus, Kalamazoo, Michigan, USA
Benjamin T. Holton, Western Michigan University Homer Stryker M.D. School of Medicine WE Upjohn Campus, Kalamazoo, Michigan, USA
Christian D. McFadden, Western Michigan University Homer Stryker M.D. School of Medicine WE Upjohn Campus, Kalamazoo, Michigan, USA
Christopher S. Keator, Western Michigan University Homer Stryker M.D. School of Medicine WE Upjohn Campus, Kalamazoo, Michigan, USA
The effects of gnawing environment enrichment (gEE) on food consumption and weight gain in mice are poorly understood. Based on a previous study conducted ~25 years ago, we hypothesized that mice provided multiple forms of gEE would increase food consumption and weight gain. We designed two projects to test the hypothesis: Project 1 examined the effects of gEE on middle-aged mice (< 18 months), whereas Project 2 examined the effects on old mice (>18 months). Project 1 included male and female mice separated into a control group (n=23), and two enriched cohorts consisting of a young adult group (~20 weeks; n=27) and middle-aged group (~42 weeks; n=24). All mice received cob bedding, a plastic arch, and nest pucks; mice in the enriched cohorts were provided 2 half gummy bones for 4 weeks, then another 4 weeks with bones plus a wooden block. Weights were recorded 3 times per week and food consumption was recorded once weekly (during cage rotation). There was no increased weight gain or food consumption (P>0.05) observed in mice given gEE. Project 2 had 3 male and 3 female cages of mice (n=4/cage) assigned to control (no gEE), enriched (2 bones) or super-enriched (2 bones plus wooden block). Aged mice given gEE for 16 weeks exhibited increased weight gain and food intake, with the gEE groups of female mice showing the greatest increases in weight gain (P< 0.0001) and long life (>30 months). Programs using old mice for neurodegenerative human disease research may find these results exciting.
Alicia Reixach, Zoetis, La Vall de Bianya, Spain
Silvia Puig, Zoetis, La Vall de Bianya, Spain
Albert Viñals, Zoetis, La Vall de Bianya, Spain
Helena Paradell, Zoetis, La Vall de Bianya, Spain
Traditional caging systems for laboratory rabbits and hamsters often restrict natural behaviors, potentially impacting animal welfare and experimental results. This project aimed to refine housing conditions by replacing cages with group housing on the floor, providing an enriched environment that better aligns with the animals' ethological needs. The transition to floor-based group housing has led to significant improvements in the physiological status of research rabbits and hamsters, facilitating more natural behavioral expression under experimental conditions and enhancing human-animal interactions.
This presentation will describe the methodology for establishing harmonized social groups, addressing key factors such as group size, space allocation and design, and environmental enrichment. Additionally, we will discuss the advantages of this approach, including enhanced well-being, reduced animal stress, and improved bonding between animals and caretakers. Some challenges associated with group housing, such as potential social conflicts, more complex animal handling, and increased husbandry workload, will also be examined. By refining housing strategies, we contribute to the ongoing evolution of ethical and effective research practices, fostering both scientific integrity and animal welfare.
PENA, EDGAR, SingHealth Experimental Medicine Centre and National Large Animal Research Facility, Singapore
Bats are being used as model for ageing and zoonotic diseases due to their extraordinary biological characteristics. In Singapore, there have been good results and potential research works on using bats for cardiovascular and metabolic research. NLARF has established the colony of local cave nectar bats (Eonycteris spelaea) in 2015, and it has grown from five to more than a hundred heads. The colony was housed in mobile cage system with limited occupancy of twenty adult bats per unit. There have been recorded fight injuries and unexplained mortalities especially after pups weaning and regrouping procedures. Despite the space constraint, the colony still thrived and has provided a jump start for infectious research.
As a step-up measure, the research and veterinary team has transformed the mobile cage to an aviary which increased the flight space of individual bat up to threefold. The large enclosure was able to combine the whole population with wider areas to fly and elevated dark roosting space. A 24-hour live camera units were deployed to monitor the behaviour of bats and social activities.
For six months of monitoring, there were significant reductions of fight injuries, pup mortalities and unexplained deaths. Despite mixing the colony , the bats were able to form their social grouping. The simplified and wider housing also provided extra convenience for the animal care staff to perform with ease the daily care and husbandry activities, saving time and disruption of colony during their resting hour on daytime.
Coleman, Kristine, Oregon National Primate Center - Oregon Health and Science University, Beaverton, Oregon, USA
MacAllister, Rhonda, Oregon National Primate Center - Oregon Health and Science University, Beaverton, Oregon, USA
Sullivan, Shelly, Oregon National Primate Center - Oregon Health and Science University, Beaverton, Oregon, USA
It is well known that researchers and care staff can be negatively impacted by the stress of caring for research animals. As such, an increasing number of facilities are offering resiliency programs. In 2020, the Oregon National Primate Research Center established a Resilience and Compassion in Research (RCR) working group, composed of clinical, husbandry, behavioral, and scientific staff, to address these issues. The RCR offers a variety of programs and approaches, including having a quiet room staff can utilize, seminars/talks that promote the value of research and/or animal welfare, and a visit from therapy llamas. To examine the efficacy of our program, we provided staff with an anonymous survey. The survey included questions to elicit feedback on each initiative and the overall program to determine what can be improved or added to maximize employee well-being (e.g., were they enjoyable, etc.). Over 120 people responded to the survey. The majority of individuals who participated in the activities rated them as enjoyable and useful to promoting their well-being. Not surprisingly, the llama visit scored highest in enjoyable. Interestingly, a number of individuals indicated they did not use one or more of the activities, either because they did not know about the activity, did not have time, or were not interested. A smaller number reported that they were discouraged from participating. The RCR utilized that information to improve the program, including ensuring that people are provided time to participate in resiliency activities.
Diego Celdran-Bonafonte, University of Arizona, Tucson, Arizona
Our animal care program has implemented an innovative 3D printing resource platform that develops, customizes, and shares a variety of 3D printed designs aimed at training and refining animal procedures. The platform employs accessible and affordable 3D printing technologies to create a library of models that help researchers and animal care staff embrace the 3Rs in a wide variety of applications.
This initiative compiles publicly available models, published models provided by their authors, models designed inhouse to provide solutions to commonly found limitations, and customized models to solve research-specific needs. We have developed several high-fidelity anatomical models, surgical and bio methods training simulators, and devices to optimize inhaled anesthesia and minimize WAGs exposure among others. Through iterative design improvements and close collaboration between veterinary professionals and researchers, we contribute together to fostering a culture of care across the entire program.
This approach has helped us significantly reduce the use of live animals and cadavers in methods and surgical training, maintaining training quality, and improving both trainer and trainee satisfaction. It has helped optimize complex surgical procedures minimizing the use of animals, contributed to reducing occupational exposure to WAGs at lab locations, and enabled safe inhaled anesthesia of rodent neonates and less common lab animal species.
Our experience demonstrates that 3D printing can effectively support the 3Rs in animal care programs, contributing to fostering a culture of care among lab animal professionals and helping set new benchmarks for ethical and cost-effective training approaches in laboratory animal science.
BEYL Alyssa
SINGER China
MCCOOL Payton
The evolution of enrichment over the past 50 years has not only enhanced animal well-being at Medtronic PRL but has also helped staff better understand and connect with our animal heroes. Implementing and maintaining enrichment in our facility did not have a clear pathway, causing the process to take longer than needed at times. Individuals could suggest new ideas, but with no clear point person, approvals from managers and veterinarians would come at separate times, and husbandry staff was left to trial during their daily cleaning. To initiate a more cohesive enrichment program at PRL, an enrichment committee was established in 2020. This diverse team of husbandry, technicians, vets, and managers frequently meet to brainstorm new ideas, trial them, and maintain both preexisting and new enrichment. A few examples include fresh produce as a healthier treat option, activities to stimulate the mind for all species, extra enrichment during quarantine to promote acclimation, and dog safe pheromones to help reduce stress. Our results are currently being compiled. Surveys have been distributed to various teams to assess whether enrichment has had a positive or negative impact on their compassion fatigue. Additionally, we are gathering data from relevant studies to evaluate whether the animals' behavior has improved during monitoring. We are seeing promising results. In conclusion, the evolution of enrichment at Medtronic PRL has changed the course of standard enrichment care. This expanding enrichment has deepened our bond with the animals and had a wholly positive impact on our facility.
SAGOR Coline, Charles River Laboratories RMS, Lyon, France
Compassion fatigue is a deep feeling of physical and emotional exhaustion that can appear while working with laboratory animals and witnessing animal suffering. There is an increase in awareness about this issue and how widespread it is in our work. Yet, it remain one of the main causes of high turn over in animal experimentation , and solutions can be hard to identify and implement.
We struggle to find the right balance between rationality and emotions, as being too emotional is often seen as a risk of bias in the scientific environment. We are judged by ourselves, our colleagues and our peers. Veterinarians and animal welfare experts have an important role to play here. By bringing their scientific, behavioral and welfare expertise, paired with their animal suffering empathy, they can become a bridge between rational and emotional people. They can help bring understanding of one another and appeased decision making on both sides, decreasing the burden of ethical dilemmas by sharing it.
Euthanasia is a perfect illustration of this challenge, systematically appearing as a major cause of compassion fatigue. Making hard decisions, especially in a context of urgency, can lead to emotional strains and doubts about the rightness of our choices, our actions. This presentation will follow three cases study (planned euthanasia, end point reached, depopulation) and tackle the ethical dilemma they generate, their consequences on everyone involved and will suggest practical solutions to reconcile the brain and the heart, to improve our methods and communication on this subject.
Mikele Simkins, DVM, DACLAM, Department of Comparative Medicine, Pfizer, Inc. Pearl River, NY. USA
Laura Singer DVM, DACLAM, Department of Comparative Medicine, Pfizer, Inc. Groton, CT. USA
Patricia Kowal MBA, MLAS, MS, LVT, RLATG, SRS, CMAR, CPIA, Department of Comparative Medicine, Pfizer, Inc. Groton, CT. USA
Dinesh Hirenallur Shanthappa DVM, MVSc, PhD DACLAM, Department of Comparative Medicine, Pfizer, Inc. Kendall Square, MA. USA
Rosemary Santos, VMD, DACLAM, Department of Comparative Medicine, Pfizer, Inc. Groton, CT. USA
Maureen Hargaden VMD, DACLAM, Department of Comparative Medicine, Pfizer, Inc. Pearl River, NY. USA
In 2023 Comparative Medicine initiated a strategy to build an active and robust Culture of Care (CoC) at all US Pfizer sites conducting animal research. It consists of three pillars: intentional, consistent progress in animal care and welfare while maintaining study quality, colleague care and wellbeing, and openness and transparency internally and with the public. A CoC survey was used to benchmark and build Pfizer's CoC strategy by informing us of our top priorities. In the area of animal welfare, we successfully initiated a program to implement non-aversive mouse handling at sites for husbandry and when possible, research procedures and are working towards evaluating digital caging for welfare benefits. Vivarium staff formed compassion fatigue teams, who educated and helped each other learn and cope with the day-to-day challenges of work in animal care and implemented unique activities at their respective sites to manage compassion fatigue such as book clubs, movies, and decompression rooms. An external licensed therapist lectured to large groups and facilitated small peer discussion groups on topics such as burnout and compassion science. Our last pillar of openness and transparency is being addressed by expanding our outreach efforts through playing our copyrighted game Long Road to Drug Discovery, sharing photos on trusted animal research advocacy websites and working to find mechanisms to increase our footprint on the corporate public website and annual report. Leadership continues to set annual performance-based goals to demonstrate our commitment to CoC and to ensure resources are allocated to advance the strategy.
Phanuphong Dungkhokkruat, CULAC, Thailand
Phit Thammanitniyom, CULAC, Thailand
Choopet Nitsakulthong CULAC, Thailand
Pigs are widely used as models in dental research due to their anatomical and physiological similarities to humans. In studies involving tooth extraction, postoperative monitoring is essential to assess pain and ensure proper recovery. Surgical sites should be examined twice daily for signs of infection or complications. Pain is associated with a reduction in feeding behavior, and pigs often exhibit a significant decrease in feed and water intake during the postoperative period, typically lasting 2–5 days. In a study on postoperative care following tooth extraction, feed enrichment was provided after the administration of antibiotics and analgesics over five days. Pain assessment was conducted using the pig grimace scale, focusing on ear position, cheek tightening, nose bulging, and orbital tightening. A score of 0 indicated the absence of these features, with ears facing forward and a smooth transition from snout to cheek. The body condition score was 3/5. Assessing pain in pigs remains challenging, as they strongly resist handling and often vocalize loudly regardless of pain status. To improve animal welfare in dental research, continued advancements in pain management strategies are necessary. Regular reassessment of care protocols and an increased awareness of key welfare concerns will contribute to more ethical and effective research outcomes.
NARAINAPOULLE Kistnasamy 'Sam', Bioculture (Mauritius) Ltd
GRIFFITHS Mary-Ann, Bioculture (Mauritius) Ltd
BABOO Eric, Bioculture (Mauritius) Ltd
LECLAIR Jasmin, Bioculture (Mauritius) Ltd
Bioculture is a company dedicated to the ethical breeding and export of Mauritian cynomolgus monkeys for biomedical research. The company was founded in 1984, pioneering the sector nationally, and has been AAALAC accredited since 2006. Since its beginnings as a small family business, the founders have fostered a Culture of Care by encouraging interaction between leadership and staff, as well as across departments in the pursuit of developing best practices aimed at maximizing animal welfare. At first, such an approach was a necessity as the knowledge base for ethical outdoor breeding of monkeys at origin was scant: learning by doing was the only way.
As Bioculture grew in size and sophistication, the organisational structure and company policies were deliberately designed to prioritize animal welfare by way of a Culture of Care. Recognizing that positive outcomes for both its bottom line and animal welfare standards depend on the values and behaviour of its people, Bioculture developed an elaborate employee welfare programme as well as community engagement and biodiversity conservation programmes to support the social and environmental ecosystems of its people. Since 2023, Bioculture has restructured its Ethical Review Committee (in lieu of IACUC) as the 'One Welfare Committee', embracing the One Health concept which posits that animal welfare depends on and influences human welfare and environmental sustainability. In this presentation, we will share Bioculture's journey of institutionalizing a Culture of Care, highlighting practical examples and lessons learnt.
HAYESHI Rose, Preclinical Drug Development Platform, North-West University, Potchefstroom, South Africa
SERAGE Irene, Preclinical Drug Development Platform, North-West University, Potchefstroom, South Africa
PHEIFFER Wihan, Preclinical Drug Development Platform, North-West University, Potchefstroom, South Africa
BESTER Cor, Preclinical Drug Development Platform, North-West University, Potchefstroom, South Africa
MINNAAR Nico, Preclinical Drug Development Platform, North-West University, Potchefstroom, South Africa
In our commitment to advancing animal care and use standards, the PCDDP implemented several key improvements in the care of laboratory mice and rats, significantly enhancing both animal welfare and program efficiency. One of the practises that has been implemented is the use of colour-coded cage cards. This system streamlines identification and management of animals, enabling quick reference to specific animal needs, reducing errors, and improving recordkeeping, which is especially critical in large-scale rodent housing environments.
In terms of animal welfare, we introduced cotton wool in the cages of nude mice and rats to provide additional warmth and comfort. This simple yet effective measure has shown to improve the well-being of animals, fostering a more comfortable and supportive environment.
A core focus of our program is the integration of animal research ethics through the 12Rs Framework, as supported by legislation. The 12Rs Framework consists of domains: Animal Welfare (Replacement, Reduction, Refinement), Social Value (Respect, Responsibility, and Regulations), Scientific Integrity (Reproducibility, Relevance, and Transferability). The intersection of the three domains leads to Righteousness, Reliability, and Reckoning. The framework provides a holistic ethical compass for experimenters. We have embedded animal research ethics into our animal handling courses, ensuring all researchers are not only trained in technical procedures but also understand the ethical responsibilities of working with laboratory animals. This has contributed to a stronger culture of care, empathy, and responsibility across our team. These strategies have collectively enhanced the welfare of our laboratory animals while promoting an ethical and efficient research environment.
Michelle E. Stewart, Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
Becky Starbuck, Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
Greg Joynson, Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
Russell Joynson, Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
Rasneer Sonia Bains, Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
Sara Well, Mary Lyon Centre, MRC Harwell, Oxfordshire, United Kingdom
Home Cage Monitoring (HCM) is increasingly used for phenotypic and welfare evaluations of Genetically Altered (GA) mice. However, these systems are often complex or require post hoc data analysis to determine phenotypes. While this information is valuable for model characterization, it may not always provide timely insights for welfare decisions. To address this, we have implemented 'sick bays' throughout our animal rooms. These are standalone home cage monitoring units that animal technicians set up and analyze in near real-time. Although there is no quantitative data analysis, the video footage of mouse behavior has proven crucial for welfare assessment. During routine checks by technicians, the mice often exhibit natural prey behavior, masking signs of pain and distress. In contrast, when observed solely through the camera, they are more likely to show authentic symptoms. Coupled with the analysis of these videos by skilled animal technicians, early indicators of pain and distress can be identified more quickly. We present examples of using these systems to monitor newly generated GA lines and to address existing welfare concerns in established colonies.
Denise Ostmeyer, Colorado State University, United States
Ashley Creighton, Colorado State University, United States
Kate Bruner, Colorado State University, United States
Expressing and receiving gratitude has measurable benefits for both personal well-being and workplace productivity. A culture of gratitude fosters stronger relationships, enhances employee engagement, and contributes to overall job satisfaction. In this presentation, we will showcase how Lab Animal Resources at Colorado State University integrates gratitude into daily operations to create a more positive and supportive work environment. We emphasize gratitude at all levels—supervisor to employee, peer-to-peer, and employee to supervisor through a variety of methods. These include public acknowledgments such as the Wall of Thanks, departmental events, celebrations, and award nominations, as well as periodic feedback surveys that not only assess different aspects of the workplace but also encourage employees to share positive feedback with colleagues and supervisors. Our methods are simple, cost-effective, and easily adaptable to other institutions. By embedding gratitude into workplace culture, we have observed tangible benefits including stronger team cohesion, improved employee retention, and higher overall job satisfaction. This presentation will provide practical strategies that can be easily implemented in other organizations. Small, intentional acts of appreciation can make a significant difference, transforming the work environment into one where employees feel recognized, supported, and inspired to contribute their best efforts.
SUSAN ROSATIa AND F. CLAIRE HANKENSON
Susan Rosati, BA, CVT, M.S.Ed., LATG, University Laboratory Animal Resources
University of Pennsylvania
Claire Hankenson, DVM, MS, MFA, DACLAM, University Laboratory Animal Resources
University of Pennsylvania
In 2021, the Division of Training, Wellness, and Education was created within University Laboratory Animal Resources (ULAR). The combined staff members for this division were from husbandry and staff training areas who were tasked with building a wellness program and advocating for employees to combat aspects of compassion fatigue and promote resilience. As part of this effort, ULAR Leadership created a Culture of Care mission statement that was approved by the Institutional Official and posted at entryways to animal facilities. An additional goal was to include researchers for improved engagement with the animal program and to foster collaboration and community within our ULAR teams. Currently, wellness events are hosted by a variety of staff from across ULAR and we highlight related outcomes and photographs in our ULAR newsletter, semi-annual staff meetings, and summer/winter celebratory events. Dedicated activities to strengthen human-animal bonding have included making treats and enrichment items and delivering them across animal housing areas, in addition to having animals create paintings that our staff have been eager to hang in work areas. Our Culture of Care includes care for animals, care for others, and care for ourselves. Through consistent messaging and encouragement of wellness education, our actions have improved engagement and a sense of community for our ULAR colleagues and the partnership with scientists. The presentation will highlight evidence-based accomplishments over the last few years for our Culture, as well as how wellness programming is designed and implemented, along with future plans to increase engagement in this initiative.
BURY Scott, Vanderbilt University Medical Center, Nashville TN, USA
SCHOENLEBEN, Aubrey, University of Washington, Seattle WA, USA
BROT, Michelle, University of Washington, Seattle WA, USA
The Compliance Use Standard Procedure (CUSP) database is a repository for standard methodologies and procedures that are being used by research labs at institutions across the United States. The benefits of using CUSP include reducing administrative burden and increasing consistency across research groups that perform similar procedures.
Submitted procedures are collated and organized in the database and available for the larger research community to peruse and potentially integrate, after they are reviewed and approved at the user's home Institutional Animal Care and Use Committee (IACUC). This project is a burden reducing initiative of the 21st Century Cures Act and is supported by the National Institutes of Health (NIH) and the Federal Demonstration Partnership (FDP). Use of this database is free of charge to users. CUSP is available to all FDP member-institutions as of February 2025 and will be available to the public later in the year.
Focused searches within CUSP can assist researchers to refine their current methodology, consider new models and options for replacement with lower species, and improve experimental design. CUSP also serves as a general resource for any researcher or veterinarian on a wide variety of practices across diverse and atypical species, enabling not only the sharing of research best practices, but also husbandry and care procedures (e.g., housing, environmental enrichment, behavioral management). Finally, CUSP encourages interaction within the research community as there is an option to share contact information for the institution contributing a procedural description.
Mohammed Zacky Ariffin, Ph.D, IACUC, National University of Singapore
Tan Yuen Peng, Ph.D, IACUC, National University of Singapore
Post-approval monitoring (PAM) is a mechanism to continuously assess compliance by the researchers to National Guidelines and approved IACUC protocols and policies. It involves observation of procedures performed by the researchers, review of records such as animal body weight, tumor size, drug administration, or an audit of the breeding cages and records.
At the National University of Singapore (NUS), PAM is conducted independently by Compliance Officers from the IACUC Secretariat in an announced or unannounced manner in the vivarium and satellite procedure rooms. The systematic approach allows identification of protocols with great potential welfare concern for PAM to be conducted. During PAM, Compliance Officers interact with researchers to nudge them towards good practices. The IACUC also encourages researchers to submit a self-report in the event of incident(s) affecting animal welfare. Together this reinforces their concept of compliance. PAM can be requested by the IACUC or veterinarians. The outcomes of PAMs are presented by the Compliance Officers to IACUC for discussion at the monthly IACUC Quorate meeting. In 2023, the IACUC Secretariat established an Education and Outreach arm to further drive efforts towards educating and enhancing awareness of animal welfare and compliance to complement PAM. Outreach can take many forms such as one-on-one meetings with researchers, Townhalls and on-boarding sessions, through newsletters, and providing guides for researchers. The primary aim of education is to develop a sense of partnership with IACUC in the NUS research community. This creates mindfulness and acceptance of compliance, self-monitoring and pre-emption of incidences to avoid non-compliance.
Bhupinder Singh, Rutgers University, USA
Jeetendra Eswaraka, Rutgers University, USA
The health and welfare of laboratory animals are crucial for ethical research and scientific integrity. Traditional health monitoring relies on daily visual assessments by trained personnel, typically conducted during rodents' inactive phase. However, these brief visual observations may fail to detect subtle yet critical health indicators, often obscured by enrichment materials. Automated home cage monitoring (AHCM), combined with machine learning (ML) algorithms, offers a promising alternative for continuous health assessment. Reduced activity is a key indicator of distress or illness in rodents, and continuous tracking enables earlier detection of health deterioration compared to conventional visual observation method. We utilized an ML-based system to generate digital alerts for potential health concerns and validated these predictions against clinical records. Our findings indicate that the ML algorithm can identify animals in distress few days before clinical symptoms or mortality. This suggests that AHCM provides a more sensitive and reliable measure of rodent health than human observation. Integrating AHCM into laboratory animal facilities can significantly enhance animal welfare by detecting subclinical conditions, enabling timely study endpoints, and improving operational efficiency. This approach represents a substantial advancement in laboratory animal welfare and research methodology.
Joseph Collins, Rutgers University, New Jersey, USA
Jeetendra Eswaraka, Rutgers University, New Jersey, USA
Rutgers University has deployed a digital home cage monitoring solution that greatly impacted their husbandry operations and improved animal welfare. Through several validation studies, the Rutgers team has shown the ability to greatly extend cage change frequencies, without negatively impacting the cage environmental conditions. Cages were observed for the duration of the studies to determine when independent observers determined cages met wet spot criteria for a cage change. Ammonia and CO2 parameters were captured and verified to fall within acceptable ranges throughout the studies. Digital parameters were collected to align with the visual observations and readings to deploy an automated process by which to drive cage changes. Automated process was implemented and augmented using user feedback (OK, too clean and too dirty) to correct and optimize. Algorithm was then implemented for all cages utilizing the cage monitoring solution and yielded a ~50% savings in cage changes needed. Since cage changes are arguably one of the most stressful activities, Rutgers was able to not only improve the efficiency of its husbandry operation, but also improve animal welfare. The team observed anecdotal evidence of this welfare improvement via an epilepsy model that was housed on the system. These animals were prone to cage change induced seizures that drastically reduced their lifespan to that of only 5-6 months. With the implemented extended cage changes, these animals were observed to live significantly longer, some even going on to live 18+ months. Rutgers is looking to further deploy this system based on these results.
Steve Gonzalez, Mike Wisnieski
Ergonomics in laboratory animal facilities is critical in ensuring worker safety, efficiency, and overall well-being. The ARES robotic system was developed to automate partial cage change procedures. It can process and handle cage bases for dumping and bedding, including rack washer applications. This system is ideal for high-throughput efforts that require 2000-8000 cages per week. The design affords a full system with operational units on both dirty and clean side of cage wash. Through its implementation, we have successfully processed well over 2000 combined caging of GM500, 1145T, and GR900 cages weekly using its customized cycles. The dirty side system takes approximately 13-25 minutes to scrape and dump cages into our compost dumpster. With our corn cob bedding, the clean side system takes approximately 12 minutes to bed caging. The rack washer application runs for roughly 7-13 minutes per cycle, supporting most of our clean caging in the vivarium. Overall, the ARES system has increased efficiency in vivarium operations, enhanced ergonomics, and reduced allergen exposure. By leveraging robotics, facilities can shift human roles from physically intensive labor to supervisory and maintenance tasks, leading to improved job satisfaction. This innovation aligns with industry trends toward automation and sustainability, setting a new standard for efficiency and worker safety in biomedical research environments.
LAHUE Karolyn G, Office of Animal Care Management, University of Vermont, Burlington VT, USA
LOVELETTE John W, Office of Animal Care Management, University of Vermont, Burlington VT, USA
WOOLSEY Joshua M, Office of Animal Care Management, University of Vermont, Burlington VT, USA
WASHINGTON Ida M, Office of Animal Care Management, University of Vermont, Burlington VT, USA
The University of Vermont (UVM) Office of Animal Care Management (OACM) continually seeks innovative methods to protect animal welfare and improve efficiencies and compliance. OACM recently converted from an outdated electronic and paper system to state-of-the-art software, resulting in improved oversight, researcher compliance, efficiencies, and concordance with IACUC animal numbers. OACM partnered with a software company to design and develop an innovative electronic animal care management system, customized for our needs, to replace an outdated inefficient system with limited capability. The new software uses QR codes on cage cards to store animal information readily accessed by tablet or mobile device. Researchers initiate animal orders, imports, exports, transfers, births, weans, and splits, and annotate cage cards electronically with pertinent information. Staff can submit queries and feedback directly to the software team. Our new software has resulted in significant improvements in animal care at UVM. Animal use counts are tracked in real-time, allowing efficient and accurate monitoring and oversight by OACM. Animal imports, exports, transfers, births, weans, and splits are recorded immediately, resulting in accurate information for census and billing. Animal use overages are detected immediately and corrected rapidly to maintain compliance. Tracking of animal locations and numbers is transparent to all stakeholders and ensures concordance with IACUC protocols. New software implementation has significantly improved UVM's Culture of Care by increasing OACM oversight capabilities, researcher awareness of animal numbers, and communication and coordination between OACM and researchers, enhancing animal welfare. OACM recommends this customizable software to institutions considering electronic animal care management.
Gianfranco Di Caro, GSK Vaccines, Siena, Italy
Marco Amadori, GSK Vaccines, Siena, Italy
Daniela Boncompagni, GSK Vaccines, Siena, Italy
Marco Martelli, GSK Vaccines, Siena, Italy
The impact of human activities on the shift in temperature and weather patterns is calling industries to accelerate the transition to net zero greenhouse gas emissions. GSK has established an ambitious program to efficiently use resources and build sustainable research and development practices. The Animal Resources Center (ARC) at the GSK Siena site has adhered to this initiative led by the Quality Control department. A Green Ambassador was appointed to catalyze the green culture and liaise with the ARC management and shopfloor to develop green initiatives, while taking into consideration animal welfare needs, quality and regulatory compliance. A forum composed of Green Ambassadors from different areas was established to discuss and monitor green projects at a larger scale within the organization. A management monitoring program was set up to track projects, run GEMBAs and escalate roadblocks.
The list of sustainability projects implemented included the revision of the sanitation procedures, the replacement of paper-based documents with electronic equivalents, the implementation of reusable gowns and PPE instead of single use items, the adoption of more integrated approaches for the management of chemicals among the quality control departments, the participation in the international freezer challenge, the installation of light- emitting diode (LED) lighting fixtures coupled with motion sensors in corridors and administrative areas to reduce electricity consumption. Our working experience illustrates the challenges addressed to reduce the environmental impact through internationally recognized accreditation programs, in a highly regulated environment, where similar needs but different scenarios may exist in research and quality control facilities.
BLAU, Christoph, Charles River Laboratories, Edinburgh, Scotland
ARCHIBALD, William, Charles River Laboratories, Edinburgh, Scotland
This Presentation will discuss how blood draws via microsampling can improve animal welfare in non-rodent species, whilst facilitating safe, low stress handling. Microsampling for Toxicokinetic / Pharmacokinetic bioanalysis is an established process that has been used at Charles River Laboratories in Edinburgh for Rat, Mouse and Rabbit studies since 2012.
We have successfully developed microsampling techniques with Hens, Non-Human Primates, and Minipigs, that use the similar equipment and processing, ensuring a consistent approach across species. Hens can be difficult to bleed as their veins can be fragile. Our technique uses gentle restraint to enable multiple sample to be taken from the veins, with minimal trauma.
Minipigs are historically sampled via the Vena Cava, whilst secured in a sling. This can be challenging as some animals may resist, occasionally leading to multiple attempts to obtain the sample. We train our Minipigs to walk to the procedure area, voluntarily walking onto a platform, which can be raised to the required height to perform sampling. The animals are gently restrained with foam pads, whilst we obtain a microsample from one of the blood vessels in the ear. On completion, the platform is lowered, and the animals walk back to their home pen.
Kate Bruner, Colorado State University, Fort Collins, Colorado, USA
Ashley Creighton, Colorado State University, Fort Collins, Colorado, USA
Julie Maynard, Colorado State University, Fort Collins, Colorado, USA
Tunneling is a method to move mice in a less stressful way than the widely used tail handling method. It reduces stress in mice by allowing them to choose to walk into the tunnels as opposed to being forcibly picked up by the tail, mimicking predator/prey behavior. We have found a cost-effective and environmentally friendly way to implement this practice.
Instead of purchasing tunnels we evaluated two no-cost options: 1. utilizing the huts already in the mouse cages, and 2. modifying broken water bottles by cutting both ends off to create a tunnel. Our facility collects broke water bottles for recycling; these were assessed for safety before modification for use with the mice. All options were tested for ease of use and mouse preference. Once we determined mouse tunnel preference and best practices, we introduced this to our animal husbandry staff and held multiple training events to learn this new technique. Staff were initially worried that tunneling would make cage changes take longer. After timing cage changes, we found tunneling takes the same or less time than moving the mice with the tail handling method. Tunneling is now a standard practice for our rodent husbandry program. Staff have appreciated this addition to our husbandry program, noting that they have noticed a positive impact for themselves and the mice. Utilizing existing huts and repurposing mouse water bottles are great no-cost options to improve mouse welfare and introduce tunneling to your rodent facilities.
Eleanor Elliott-Brown, Charles River Laboratories Portishead United Kingdom
Maisie Colwill, Charles River Laboratories Portishead United Kingdom
Jenna Lamond, Charles River Laboratories Portishead United Kingdom
The standard conception is that male mice, with high levels of testosterone, will act aggressively towards cage mates resulting in high stress with potentially deadly side effects. However, it has also been cited that social housing in rodents aids recovery time post-procedures with male mice showing less signs of stress when housed with conspecifics. Due to conflicting literature, when 12 male mice reported as being 'highly aggressive' arrived singly housed to the facility the initial reaction was to follow protocol, males stay as they arrive. However, a different route was considered based on personality. All 12 mice were tail marked and placed into a playpen consisting of treats and all neutral enrichment and bedding to prevent territoriality. Using a basic ethogram, categorising positive and negative interactions the mice were continuously monitored, peer interactions were tallied and mice were paired with whom they had the most positive interactions. All cages were returned to their home rack and checked frequently. Throughout PM checks, fighting amongst 3 cages was observed but this settled by ~8h post-allocation. In addition to this, no further fighting was observed for the remainder of acclimatisation and study duration. Acclimatisation also included human interactions whereby they willingly interacted with handlers. Through acclimatisation pre-study, the mice were taught being handled was a positive experience meaning postsurgery they showed less aversion to humans and procedures. Allowing mice to select cage mates that had complimentary personalities allowed for better recovery and reduced mortality associated with non-study specific observations; less stress=better science.
Hee Yeon Jeon, Veterinarian, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Ji-Seung Ko, Veterinarian, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Young-Shin Joo, Attending-Veterinarian, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Yu jin Lee, Laboratory Animal Technician, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Selim Yang, Laboratory Animal Technician, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Eun Hye Lim, Laboratory Animal Technician, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Chang Hyun Jee, Laboratory Animal Technician, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Ji Hoon Yeo, Laboratory Animal Technician, Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea
Seong-Beom Lee, IACUC Chairman, Department of Pathology, College of Medicine, The Catholic University of Korea
Chan Kwon Jeong, Laboratory Animal Research Center Director, Department of Hospital Pathology, College of Medicine, The Catholic University of Korea
Department of Laboratory animal, College of Medicine, Songeui Medical Campus & Animal Research Support Unit, Laboratory Animal Research Center, The Catholic University of Korea has been operating a bulletin board called Sharing Animal-derived Resources Board on our website since 2021. This bulletin board is used as a space where resources derived from laboratory animals(organs, blood, etc.) can be shared. It aims to enable researchers to share animal-derived resources to reduce unnecessary use of laboratory animals, experiment period, and cost, and lead to various research results. In the case of a researcher who wants to be shared resources, they can write the information about the necessary animal species, strain, and organs, as well as information about the experiment to do. For researchers who want to share their resources, they post information about animal species, strain, number of animals, experimental process, euthanasia schedules, and organs that can be shared. Researchers can communicate with each other directly through this boards, and LARC staff can review the content and match it appropriately. Researcher can share the animal-derived resources that are not necessary for their research once they have finished their experiment. These could be important resources for other researchers who need that sample. Through this bulletin board, resources can be shared without conducting animal experiments, which helps replace experiments(replacement) and reduce the number of animals used(reduction). This provides an efficient research environment for researchers and enables the implementation of ethical experiments that meet the 3R principles of animal experiment.
Xiaoqing Chi, Center for Drug Safety Evaluation and Research of Zhejiang University Hangzhou, Zhejiang, Chin
Yi Zhang, Center for Drug Safety Evaluation and Research of Zhejiang University Hangzhou, Zhejiang, Chin
Yujie Qian, Center for Drug Safety Evaluation and Research of Zhejiang University Hangzhou, Zhejiang, Chin
Qinjie Weng, Center for Drug Safety Evaluation and Research of Zhejiang University Hangzhou, Zhejiang, China
The 3Rs (Replacement, Reduction, and Refinement) are fundamental principles guiding ethical animal research. While significant progress has been made in reducing animal use, implementing effective refinements without compromising research quality remains challenging. Our institution developed a data-driven refinement strategy integrating real-time physiological monitoring, AI-assisted behavioral analysis, and statistical modeling to optimize study design and animal care.
To enhance Refinement, we implemented telemetry-based real-time monitoring, collecting continuous physiological data on stress levels, temperature fluctuations, and activity patterns. This allows for proactive adjustment to study conditions and humane endpoints refinement. Additionally, an AI-assisted behavioral analysis system was integrated into Y-maze and Open Field Tests, detecting subtle stress indicators to improve housing conditions and handling procedures.
For Reduction, we applied statistical modeling and meta-analysis to optimize experimental design, ensuring that each study achieves maximum statistical power with the minimum number of animals required.
Over the past three years, these refinements have led to a 20% reduction in animal use while improving study reproducibility and welfare outcomes. The telemetry system allowed for early detection of distress, resulting in timely interventions. The AI-assisted behavioral assessments improved the understanding of subtle stress behaviors, leading to better housing and handling modifications.
By integrating real-time physiological monitoring, AI-driven behavioral analysis, and statistical modeling, we have demonstrated how data-driven refinements can enhance both animal welfare and research integrity. This approach exemplifies how technological advancements can effectively implement the 3Rs, fostering a culture of care and setting new standards for ethical animal research.
Zongcheng Wu, SYRICIT (Nantong) Co., Ltd., Nantong, Jiangsu, P.R. China
Yuyan Zhao, SYRICIT (Nantong) Co., Ltd., Nantong, Jiangsu, P.R. China
Lixiang Ren, SYRICIT (Nantong) Co., Ltd., Nantong, Jiangsu, P.R. China
Traditional inhalation toxicity testing relies heavily on animal models, causing ethical and welfare concerns due to prolonged exposure, significant distress, and endpoints involving mortality or severe reactions. Complete replacement by in vitro methods remains impractical, underscoring the need for scientifically rigorous alternatives that minimize animal use and suffering.
We developed an integrated inhalation toxicity assessment approach combining quantitative structure-activity relationship (QSAR) analysis, computational toxicology, in vitro experiments, and machine learning. QSAR models (ACToR, QSAR Toolbox, TEST) predicted toxicity based on structural descriptors. Computational fluid dynamics (CFD) and Multiple-Path Particle Deposition (MPPD) simulated aerosol behavior and deposition. Physiological pharmacokinetic (PBPK/PD) models used species-specific data for precise dose predictions. A hybrid machine learning framework combining QSAR, CFD/MPPD, PBPK/PD, and in vitro datasets was validated rigorously.
QSAR models predicted toxicity endpoints accurately (92%), closely aligning with experimental data. Multiscale modeling showed a 95% correlation with in vitro aerosol deposition and pharmacokinetics. The hybrid machine learning model achieved 88% accuracy, outperforming traditional methods by 22%, reducing false positives by 30%, and decreasing animal usage by 42%. Validation across 10 independent tests confirmed consistency (80%), significantly improving animal welfare.
Our study introduces an advanced, ethical inhalation toxicity assessment strategy that integrates QSAR, computational models, in vitro approaches, and machine learning. This approach effectively reduces the use of animals (especially under the requirements of the Chinese inhalation toxicity testing standards), improves the accuracy of predictions, and meets ethical standards, making a significant contribution to sustainable and welfare-focused toxicology practice.
GENS Lena, AO Research Institute Davos, Switzerland
TAPIA-DEAN James, AO Research Institute Davos, Switzerland
ARENS Daniel, AO Research Institute Davos, Switzerland
ZEITER Stephan, AO Research Institute Davos, Switzerland
Communication is essential for fostering a Culture of Care in every animal care and use program. A core element of this culture is the commitment to open communication at all levels of the program. By implementing various initiatives that promote openness, transparency and inclusivity, we aimed to establish a more supportive and empathetic environment enhancing employee and animal welfare.
Our initiatives incorporate hosting an open-door day, where direct connection and exchange between staff members and the public fosters a sense of trust and transparency. Inviting employees' children into the workplace gives them a unique, firsthand experience of animal research, giving them a chance to develop and better understanding of the important work being done. Personal meetings with the regulatory oversight body have streamlined the ethical review process, making ethical discussions more efficient while strengthening collective responsibility for animal welfare. Additionally, presenting research projects to animal caretakers before the start and also presenting the results after completion of the project not only deepens their understanding but also allows them to give valuable input, ensuring that their contributions are truly recognized. Through these initiatives, we have created a collaborative culture of care that emphasizes the critical role of communication and the importance of every individual's contribution to animal welfare.
Saifon Sreechomphoe, Chulalongkorn University Laboratory Animal Center (CULAC), Thailand.
Choopet Nitsakulthong D.V.M.2, Chulalongkorn University Laboratory Animal Center (CULAC), Thailand.
Personnel involved in animal care, particularly those handling equipment for animal housing, are more likely to experience ergonomic injuries compared to other types of occupational illnesses. This is because their tasks often involve handling heavy objects or performing repetitive work for extended periods, which can lead to injuries in the shoulders, back, and hips. To address these issues, occupational health professionals have implemented Workplace Design strategies as follows:
1. Optimizing the workspace layout
2. Alternating tasks
3. Implementing warning signs and ergonomic guidelines.
Additionally, infection prevention measures in the laboratory have been introduced. These include providing educational materials and introductory training sessions for personnel before they begin working with pathogens. The goal is to ensure they understand the risks and necessary precautions before starting their tasks. Applying these methods can help reduce ergonomic injury risks and create a safer work environment, particularly for those who frequently lift heavy objects, maintain static postures for extended periods, or handle infectious agents. Following the implementation of the work method promotion activities, a satisfaction survey was conducted among employees exposed to ergonomic risks and laboratory infections. The survey results indicated that these ergonomic and infection prevention measures significantly improved workplace safety. Respondents also expressed a strong commitment to guiding their colleagues and subordinates on proper lifting techniques if incorrect practices were observed. Overall, the satisfaction level was rated as
highest satisfaction across all areas of evaluation.
MORENO CARRASCOSA Josep, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain
PEÑAFIEL CENTELLES Marina, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain
LUQUE RAMÍREZ Lourdes, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain
WILLAMIL DOS SANTOS Joseane, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain
FERNÁNDEZ BLANCO Joan Antoni, Barcelona Biomedical Research Park (PRBB), Barcelona, Spain
The Barcelona Biomedical Research Park (PRBB) houses multiple institutions that independently manage Occupational Risk Prevention (ORP) for their personnel. The most recent AAALAC visit highlighted opportunities to enhance ORP coordination across PRBB institutions. In response, the Animal Facility spearheaded a collaborative initiative with ORP and HR departments to streamline communication channels and develop a comprehensive approach to task-specific risk management within the facility. Key initiatives included active participation in the Inter-institutional Commission on Prevention of Occupational Risks (CIPRL) and close collaboration with the Biosafety Committee. These efforts led to the development of standardized documents summarizing critical biosafety and chemical risk data. Communication improvements were achieved through established channels such as the Animal Facility Users Committee and the PRBB Residents Portal. Additionally, SmartOSH was employed to share technical instructions, safety data sheets, and product specifications across departments.
Results demonstrated significant improvements in inter-departmental communication and unified documentation. This facilitated comprehensive risk assessments across diverse roles within the facility, including subcontracted cleaning staff, animal caretakers, and institutional researchers. A Biosafety Committee-developed form further streamlined access to biosafety and chemical risk information, providing the animal facility personnel and ORP services with summarized and direct information regarding chemical and biological risks associated with research projects conducted at the PRBB animal facility.
In conclusion, addressing AAALAC recommendations following their visit has led to enhanced communication pathways and robust control measures for biological and chemical risks. These advancements exemplify a proactive approach to fostering a Culture of Care through improved coordination and transparency across PRBB institutions.
LAFAUCE, Erica, Novartis Biomedical Research, Cambridge USA
PHUNG, Elizabeth, Novartis Biomedical Research, Cambridge USA
HAHN, Ryan, Novartis Biomedical Research, Cambridge USA
SHOWKAT, Nazaf, Novartis Biomedical Research, Cambridge USA
KOEHL-CARABETTA, Kristin, Novartis Biomedical Research, Cambridge USA
VARRONE, Georgianna, Novartis Biomedical Research, Cambridge USA
SPENCE, Cheryl, Novartis Biomedical Research, Cambridge USA
BULLARA, Domenico, Novartis Biomedical Research, Cambridge USA
GASKILL, Brianna, Novartis Biomedical Research, Cambridge USA
IBEBUNJO, Chikwendu, Novartis Biomedical Research, Cambridge USA
GROWCOTT, Ellena, Novartis Global Health, Cambridge, USA
BROTCHIE-FINE, CeCe, Novartis Ethics Risk & Compliance, Cambridge USA
Compassion fatigue (CF) and compassion satisfaction (CS) are concerns for employee wellbeing, animal welfare and retention of staff. CS is a driving force for many who work in the lab animal field but is difficult to measure. While many exhibit high empathy and caring traits, these are also a risk factor for developing CF. CF is a difficult topic for many people to discuss and recognize, especially in a professional setting. There is often perceived stigma around expressing concerns for one's mental well-being, as well as consideration that doing so may impact career development and progression. Institutional support to uphold a positive ethical environment can counterbalance some of these impacts. A Culture of Care team was formed to evaluate best strategies to mitigate CF and increase CS. Initial activities have included benchmarking at the R&D headquarters site, rollout of resources to staff, and presentations to leadership and associate teams. The next phase is a transition into a global program across all sites with in vivo activities. Considerations include local site culture, collaboration with established CF/CS programs and supporting each site through centralized executive sponsorship. We acknowledge that each location has a distinct culture and not all resources or methods may be met with the same enthusiasm. Therefore, each location is provided with guidance and resources to develop a self-owned and driven program connecting to the wholistic Culture of Care.
OWEN Emma, University of Manchester, Manchester, UK
Public support for using animals in medical research remains strong, but trust in the organizations conducting such research has often been lacking. A 2018 UK Ipsos Mori poll found that only 30-40% of the public considered these organizations to be reliable sources of information. Years of animal rights protests—some of them aggressive—drove scientists into hiding, hindering efforts to develop life-saving medicines and technologies. That dynamic shifted in 2014 with the launch of the Concordat on Openness on Animal Research, the world's first transparency agreement. As one of its original signatories, we have witnessed firsthand the benefits of greater transparency—not only for science and scientists but also for the wider public, who ultimately benefit from new treatments and medical advancements. Even the animals themselves have gained from improvements in care and welfare.
The University of Manchester has become one of Europe's most recognized institutions for its communications about animal research. Our commitment to openness earned us official recognition as a 'Leader in Openness' in 2019. Through award-winning initiatives—including our website, social media, public debates, and engagement with young audiences—we have fostered meaningful discussions and shifted perspectives. By collaborating with internal and external stakeholders, we have cultivated a culture of transparency that has strengthened both public trust and our institutional culture of care.
Solenn Percelay, TransCure bioServices
Floriane Marzin, TransCure bioServices
Noémie Spinelli, TransCure bioServices
Sebastien Tabruyn, TransCure bioServices
The concept of Culture of Care is increasingly recognized as essential for improving both animal and human welfare. One of the key elements, compassion satisfaction, is promoted through social support. However, this can be challenging to cultivate outside the lab due to people's apprehension about reactions to the sensitive topic of animal experimentation.
At TransCure BioServices, we are committed to educating and raising awareness among our entire team about why and how animals are used in research, as well as the strategies for discussing this topic with family, friends, and the general public. This ongoing education helps to increase public openness and reduce the fear of external communication, making it easier to implement transparency initiatives that are fully supported by our staff. These actions yield positive outcomes, fostering a sense of pride, enhancing both the company and the profession, and improving communication skills. As a result, our team has developed strong social support, with everyone now feeling comfortable discussing animal experimentation, as evidenced by an internal survey. The virtuous circle is completed with compassion satisfaction, which in turn strengthens our ability to engage in public openness.
Therefore, our culture of care program helps us increase openness and transparency, with initiatives such as welcoming interns, allowing cameras in our animal facility, permitting visitors to tour the institution and observe our humanized mice in oncology studies, etc. Our commitment to openness, combined with staff awareness, fosters both human welfare and transparency.
MINTZ Dvir, Technion (IIT), Haifa, Israel
The ongoing conflict in Israel since October 2023 presented significant challenges for maintaining operations at the Technion's animal facility. This presentation outlines the strategies and protocols implemented to ensure animal welfare, staff safety, and operational continuity under extreme circumstances. To safeguard animal welfare, contingency plans were activated, securing essential resources such as food, water, and veterinary care, while monitoring systems allowed continuous oversight of animal health and environmental conditions despite restricted physical access. Staff safety was prioritized through adaptive scheduling, flexible working arrangements, and mental health support programs, ensuring reduced exposure to potential threats. Operational continuity was ensured through innovative problem-solving, including stockpiling essential supplies, securing backup power sources, and establishing redundant communication lines. Collaborations with local authorities ensured the facility remained functional, even during prolonged disruptions. The facility's commitment to maintaining research integrity was unwavering, with all emergency actions aligned with AAALAC standards and ethical guidelines. Compliance audits and comprehensive documentation facilitated the resumption of research with minimal disruption, preserving the validity of ongoing studies. The insights gained during this period emphasize the critical importance of proactive planning, technology adoption, and team resilience. These lessons offer a framework for animal research facilities worldwide, emphasizing the need for adaptable crisis management approaches that ensure the welfare of animals, the safety of staff, and the continuity of research programs during times of crisis.
Brian W Gibson, DVM, DACLAM, Baylor College of Medicine, Center for Comparative Medicine, Houston, Texas
Rebecca S Schwiebert DVM, PhD, DACLAM, Baylor College of Medicine, Center for Comparative Medicine, Houston, Texas
The 8th edition of the Guide sets the expectations for researchers conducting survival surgery, including training in aseptic technique, gentle tissue handling, appropriate use of instruments, effective hemostasis and correct use of suture material and patterns prior to beginning work. Our institution noted an increase in serious surgical complications in rodents being reported to the IACUC, and review of the surgical complications indicated a failure to formally train all individuals to correctly perform surgery. Training was mandated for all individuals performing rodent surgery, and upon review, it was determined that we had approximately 715 individuals to train in a three-month time frame. The major limiting factor in providing such training was a training staff of three individuals. To leverage personnel limitations of the training staff, it was decided that one individual from each of the labs would be designated as the Training Ambassador (TA), would receive intensive training and proficiency assessment, and would then train their team members in the correct conduct of survival surgery. The TA program allowed us to effectively train a relatively large number of people in the three-month period mandated by the IACUC, AV, and IO. Ultimately, we were able to see a dramatic decrease in surgical complications. Ongoing use of the TA program has allowed us to leverage limited training staff to provide and maintain institutional-wide rodent surgery training. Details of methods used and training content will be provided.